Burkholderia gladioli, including a reducedgenome symbiont thought. Burkholderia glumae is a gramnegative soil bacterium. Case reports nusa matijasic 1, natasa andrijasevic2, amarela lukic grlic1,3, iva topalusic, irena ivkovic jurekovic1,4, marta navratil1,4. Therapeutic options are limited owing to high levels of resistance of the organism, either intrinsic or acquired, to many antimicrobial agents. First report of bacterial panicle blight of rice caused by burkholderia gladioli in ecuador. The bacterium burkholderia gladioli lives in specific organs of a plantfeeding beetle and defends the insects eggs from detrimental fungi by producing antibiotics. Speciesspecific pcr as a tool for the identification of burkholderia.
The vast majority of published clinical data on burkholderia infection in individuals with cf is comprised of uncontrolled, anecdotal, andor single center experiences, and no consensus has emerged regarding treatment. But a newly discovered natural antibiotic produced by bacteria from the lung infection in a cystic fibrosis patient could help fight these infections. All the information, content and live chat provided on the site is intended to be for informational purposes only, and not a substitute for professional or medical advice. Identity and origin of bacterial strains used in this study. Burkholderia pseudomallei infection in us traveler. The nomenclature of burkholderia gladioli, which is distinct from the burkholderia cepacia complex, has evolved from pseudomonas marginata and pseudomonas gladioli to its current designation. Transmissibility and infection control implications of.
They are closely related to burkholderia cepacia complex, which are well known for. Burkholderia gladioli is a species of aerobic gramnegative rodshaped bacteria that causes disease in both humans and plants. Bacterialfeeding nematode growth and preference for. Gladioli was isolated for the first time in 1921 mcculloch, 1921, and is normally pathogenic on gladiolus spp. Burkholderia cepacia oxidase test burkholderia cepacia infection treatment download here free healthcaremagic app to ask a doctor. Onion is a model plant used to test the pathogenicity of burkholderia species and b. Little is known about the regulation of virulence factor and toxin production in bgc, and studies in this bacterium have been hampered by lack of genetic tools. Burkholderia gladioli simple english wikipedia, the free. Burkholderia pseudomonas gladioli is a gramnegative bacillus in the pseudomallei group of pseudomonads that is known primarily as a plant pathogen.
Severe burkholderia pseudomonas gladioli infection in. Burkholderia pseudomonas mallei material safety data. A collection of 1,0 bacterial isolates obtained from the poplar stems in 9 regions of china. Information and translations of burkholderia gladioli in the most comprehensive dictionary definitions resource on the web. Cf patients with the aim of controlling patienttopatient transmission 57. The identity and characterization of pathogens associated with this disease was unclear. Nosocomial acquisition of burkholderia gladioli in patients with. Burkholderia gladioli are ubiquitous gramnegative bacteria that are able to occupy diverse environments including soil, plants and the respiratory tract of humans. Establishment of a comprehensive antibiotic susceptibility profile showed that. The researchers discovered that one species, burkholderia gladioli, which was isolated from the sputum of a child with cystic fibrosis, produces an antibiotic called gladiolin.
Melioidosis is an infection with clinical manifestations ranging from skin abscess to overwhelming sepsis and death. Bronchiolitis and bacteraemia caused by burkholderia. The name melioidosis also known as whitmore disease, rangoon beggars disease, vietnamese time bomb is a combination of greek words melis meaning distemper of assess and eidos meaning resembles glanders. Burkholderia gladioli a predictor of poor outcome in.
Burkholderia is a genus of proteobacteria whose pathogenic members include the burkholderia cepacia complex which attacks humans and burkholderia mallei, responsible for glanders, a disease that occurs mostly in horses and related animals. Water soaked and very pale brown lesions appeared on the internal scales of diseased onion bulbs. To atcc valued customers, atcc stands ready to support our customers needs during the coronavirus pandemic. Humans and animals are believed to acquire the infection by inhalation of contaminated dust or water droplets, ingestion of contaminated water, and contact with contaminated soil, especially through skin abrasions. Burkholderia a genus of gramnegative, aerobic, rodshaped bacteria. Burkholderia images partners infectious disease images. Burkholderia gladioli is a species of bacteria that needs oxygen and is shaped like a rod. Poplar canker is a kind of serious disease of poplar branches in china and all over the world. Burkholderia cepacia complex and burkholderia gladioli. It is caused by burkholderia pseudomallei, a gramnegative, saprophytic bacillus found in soil and water.
Inhaled aztreonam for chronic burkholderia infection in. Although b gladioli have traditionally been recognized as plant pathogens, they are increasingly being reported to cause infections in humans. Burkholderia gladioli colony morphology doctor answers on. Dear editor, the gramnegative bacillus burkholderia gladioli is known to be a causative pathogen of pneumonia in cystic fibrosis 1,2. In the edinburgh adult cystic fibrosis clinic, spread of an epidemic strain resulted in approximately 70% b cepacia colonization over a sixyear period. This name became validly published when it appeared on validation list no. Jan 26, 2012 humans and animals are believed to acquire the infection by inhalation of contaminated dust or water droplets, ingestion of contaminated water, and contact with contaminated soil, especially through skin abrasions. Oct 25, 2018 burkholderia cepacia is an aerobic gramnegative bacillus found in various aquatic environments.
We report two cases of pneumonia, one accompanied by septicemia, caused by b. Burkholderia cepacia complex infectious disease and. It is found in soil, water, the rhizosphere, and in many animals. However, it is not clear if longdistance dissemination can occur. However, due to the high degree of phenotypic similarity between this species and closely related species in the burkholderia cepacia complex, accurate identification is difficult. Microbiological and epidemiological features of clinical. The objective of the present study was to characterize burkholderia spp. Speciesspecific pcr as a tool for the identification of. Incorrect identification of these species may have serious repercussions for the. Burkholderia pseudomallei is a gramnegative, rodshaped, motile, soildwelling bacterium. The genus burkholderia is widespread in diverse ecological niches, the majority of known. An international team of researchers have discovered a remarkable microbe with a jekyll and hyde character. These isolates were pathogenic and induced symptoms of seedling rot, grain rot, and leafsheath browning in rice.
First report of bacterial panicle blight of rice caused by. Burkholderia gladioli is one of the causal agents of bacterial panicle blight of rice bpb. Horizontal gene transfer to a defensive symbiont with a. Lab testing reported in the journal of the american chemical society shows that the compound is active against multidrug. To describe the microbiology and potential virulence factors of burkholderia cepacia.
Preference plates were prepared with opposing 30l spots of a b. Nosocomial acquisition of burkholderia gladioli bacteremia in. Bacillus croci is gram negative, faculta tively anaerobic with peritrichous flagella, pathogenic on crocus sativus and the causal agent of rot lesions mainly on tubers. Describe the normal sources habitat for pseudomonas aeruginosa, burkholderia cepacia, burkholderia pseudomallei, and burkholderia mallei, including the routes of transmission. Burkholderia gladioli, primarily known as a plant pathogen, is involved in. Matijasic n, andrijasevic n, grlic al, topalusic i, navratil m, et al. Chryseobacterium respiratory tract infections in patients with cystic fibrosis. Burkholderia gladioli, which was isolated from the sputum of a. Identification and enzymatic characterization of acid. Identify the factors that contribute to the pathogenicity of p. People can get melioidosis through direct contact with contaminated soil and surface waters.
In the present study, the first respiratory isolates recovered from 14 french patients with cf and 4 french patients without cf, identified by 16s rrna gene analysis, were tested for growth on b. Nov 16, 2010 burkholderia gladioli pathovar cocovenenans bgc is responsible for sporadic foodpoisoning outbreaks with high morbidity and mortality in asian countries. Rice plants with bacterial leafsheath browning and grain rot were observed in fukuoka prefecture in japan during the autumn seasons of 1995 and 1996. Mar 18, 2009 burkholderia gladioli, primarily known as a plant pathogen, is involved in human infections, especially in patients with cystic fibrosis cf. Mahenthiralingam e, bischof j, byrne sk et al 2000 dnabased diagnostic approaches for identification of the burkholderia cepacia complex, burkholderia vietnamiensis, burkholderia multivorans, burkholderia stabilis, and burkholderia cepacia genomovars i and iii. Occurrence of bacterial soft rot of onion plants caused by. Burkholderia stagnalis is a bacterium from the genus of burkholderia. During this period, there were 122 infections in 107 patients.
Paraburkholderia megapolitana is a gramnegative, catalase and oxidasenegative aerobic, nonsporeforming, bacterium from the genus paraburkholderia and the family burkholderiaceae which was isolated from moss of poor nutrient plant communities from the southern coast of the baltic sea in germany. At one time, most of the species belonging to the genera brevundimonas, burkholderia, ralstonia, and acidovorax were members of the genus pseudomonas. Pdf burkholderia gladioli and pseudomonas marginalis. Burkholderia cepacia is an aerobic gramnegative bacillus found in various aquatic environments. Plant strains were divided into pathovars alliicola and gladioli. B cepacia is an organism of low virulence and is a frequent colonizer of fluids used in the hospital eg, irrigation solutions, intravenous fluids. Bacterial panicle blight bpb is among the three most limiting rice diseases in louisiana and the southern united states. Here we study the prevalence, dynamics, and strain diversity of burkholderia gladioli bacteria in lagria beetles, a recently described protective symbiosis involving vertical transmission and.
Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. Burkholderia gladioli pathovar cocovenenans bgc is responsible for sporadic foodpoisoning outbreaks with high morbidity and mortality in asian countries. Burkholderia gladioli was first described as a phytopathogen and later associated with certain pulmonary infections. Burkholderia gladioli was first identified as a plant pathogen in gladiolus, iris and rice, but this. An unusual burkholderia gladioli double chaininitiating nonribosomal. Melioidosis is highly endemic to southeast asia and northern australia, and endemic to the indian subcontinent, southern china, hong kong, and. Methods for genetic manipulation of burkholderia gladioli. Burkholderia fungorum is presently being sequenced by the doe joint genome institute. It can also live in symbiosis with plants and fungi and is found in soil, water, the rhizosphere, and in many animals.
Identification and characterization of burkholderia isolates. The taxonomy of burkholderia formerly pseudomonas cepacia complex currently consists of 18 distinct bacterial species with a wide environmental distribution in soil and plants, especially the underground stem rhizome 63, 73. Organisms in these genera have many similar characteristics. Definition of burkholderia gladioli in the dictionary. They are closely related to burkholderia cepacia complex, which are well. Inhaled aztreonam for chronic burkholderia infection in cystic fibrosis. General information about burkholderia gladioli pv. Bacteria from cystic fibrosis patient could help thwart. Bacteria from cystic fibrosis patient could help thwart antibioticresistant tb. While a few cases have been documented, contaminated soil and. Nosocomial acquisition of burkholderia gladioli bacteremia in two children without documented cystic fibrosis and immunedeficiency. It was first recognized as a plant pathogen of gladiolus and iris species. It is very rare for people to get the disease from another person.
In the summer of 1999, disease symptoms of unknown origin were observed on onion bulbs in the fields and storage houses in korea. Burkholderia gladioli basonym, pseudomonas gladioli or pseudomonas marginata was originally described in 1921 as a phytopathogen of gladioli and other flowers. Factors associated with transmission of burkholderia cepacia outside of hospital govan et al 17 provided further evidence to support transmission through social contact. Currie 221 t he genus burkholderia is currently composed of many species, but only three are notable pathogens for humans or animals. It was first recognized as a plant pathogen of gladiolus and iris species 1. Therapeutic options for burkholderia cepacia infections. These pathogen safety data sheets, regulated under workplace hazardous materials information system whmis legislation, are produced for personnel working in the life sciences as quick safety reference material relating to infectious microorganisms. Burkholderia cepacia infection treatment download here free healthcaremagic app to ask a doctor all the information, content and live chat provided on the site is intended to be for informational purposes only, and not a substitute for professional or medical advice. The bacterium burkholderia gladioli lives in specific organs of a. Burkholderia gladioli and pseudomonas marginalis pathogens of leucojum aestivum. Burk isolates were obtained from 44, 41, 40, and 35 azli. Burkholderia gladioli has been reported as colonizing the airways of patients with cystic.
Bacterial soft rotting was more common than scale discoloration. Burkholderia gladioli, primarily known as a plant pathogen, is involved in human infections, especially in patients with cystic fibrosis cf. It causes disease in humans and plants and can live in symbiosis with plants and fungi. Burkholderia cepacia complex bcc is an important group of pathogens affecting patients with cystic fibrosis and chronic granulomatous disease as well as immunocompromised and hospitalised patients. In china, the poplar canker is mainly caused by three pathogens of cytospora chrysosperma, phomopsis macrospora and fusicoccum aesculi, which is hard to control.
This research details studies carried out on the pathogens causing bpb on rice in louisiana and other rice producing southern states. Burkholderia gladioli colonizes the respiratory tracts of patients. The species burkholderia gladioli was originally described by yabuuchi et al. Members of the genus burkholderia are metabolically versatile gramnegative organisms occupying diverse ecological niches including soil, plants and human respiratory tracts. Symptoms of bacterial panicle blight include seedling blighting and sheath rot in addition to panicle blighting, which accounts for most of the damage from this disease. Straina host area of origin year reference burkholderia gladioli distefb strelitzia reginae italy 2006 cirvilleri et al. Population growth plates were prepared with a central 30l spot of. Nosocomial acquisition of burkholderia gladioli bacteremia. Evidence suggests that patienttopatient transmission of the organism. Chryseobacterium respiratory tract infections in patients. Burkholderia gladioli colony morphology doctor answers. The compound belongs to the same structural class as etnangien, another antibiotic that has been investigated for its ability to jam bacterial cell machinery. Describe the normal sources habitat for pseudomonas aeruginosa, burkholderia cepacia, burkholderia pseudomallei, and burkholderia mallei, including the routes of transmission 2. Organisms in this genus had originally been classified as members of the pseudomonas genus but overwhelming biochemical and chemical findings indicated the need to separate them from other pseudomonas species, and hence, this new genus was created.
Genome characteristics of burkholderia symbiont lvstb, its relative b. Burkholderia pseudomallei infection in us traveler returning. Burkholderia gladioli was described as a plant pathogen, and it is a rare cause of infection in humans that is primarily associated with human pulmonary infections, such as chronic granulomatous. Pseudomonas, burkholderia, and similar organisms clinical gate. Identification and characterization of burkholderia.
Burkholderia pseudomallei, causative agent of melioidosis. Symbiont dynamics and strain diversity in the defensive. Conversely, burkholderia pseudomallei is the causative agent of melioidosis, as disease that harms both humans and animals, as well as septicemia and pneumonia in susceptible individuals godoy et al. Burkholderia gladioli and species in the burkholderia cepacia complex are amongst the many opportunistic bacterial pathogens that cause respiratory infections in individuals with cf. Burkholderia gladioli colonizes the respiratory tracts of patients with cystic fibrosis and chronic granulomatous disease.
Isolation and characterization of a new burkholderia. Initially considered a harmless commensal colonizing the respiratory tracts of cystic fibrosis cf patients, b. Bcc and burkholderia gladioli infections in the childrens and adult clinics in vancouver between 1981 and may 30th, 2011. Melioidosis is an infection caused by the motile, an opportunistic, aerobic gramnegative bacterium burkholderia pseudomallei. Burkholderia is a large genus that contains pathogenic species as well as many that. The organism is found in soil and water in tropical climates such as, southeast asia and northern australia.
1311 635 726 1222 1571 366 5 372 1633 510 628 1579 748 880 1152 333 713 217 758 654 1049 1611 601 1555 135 591 1345 1269 389 212 128 1187 629 1416 104 211 389 785